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Runs were carried out at 23°C on a Shimadzu (Kyoto, Japan) LC-10AS HPLC at a flow rate of 0 home horizon loan new .
American Institute of Physics, New York home horizon loan new .
The PTS involves a cascade of phosphoryl-transfer steps from phosphoenolpyruvate (PEP) via phosphointermediates of the general cytosolic non-sugar-specific phosphotransferases enzyme I (EI) and the histidine-phosphocarrier (HPr) to sugar-specific enzyme II permeases home horizon loan new .
Science 2003; 302:1560-1563(30) Kunisaki Y, Masuko S, Noda M, et al home horizon loan new .
Optical spectroscopy to characterize protein conformation and conformational changes home horizon loan new .
To compare the activation energy E^sub a^ of disk denaturation for different proteins, we recorded T-jump CD data of apoA-1:DMPC disks (Fig home horizon loan new .
These may or may not be identified as intermediate states, depending on their occupancy lifetime home horizon loan new .
Disulphide-bonded intermediate on the folding and assembly pathway of a non-disulphide bonded protein home horizon loan new .
When a solution that undergoes LLPS is cooled, the phase with higher concentration always reaches its crystallization temperature first home horizon loan new .
However, because we have compared results across a series of molecules where the RNA sequence (and secondary structure) in the vicinity of the dyes has been held constant, the differences between the characteristics we observe are related primarily to tertiary structural differences between the constructs home horizon loan new .
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