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. The dielectric spectra of protein aqueous solution have usually manifest at least three relaxation processes called delta, and gamma (Grant et al
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. In this research, the sequential events in ACYI thermal denaturation were investigated by a combination of spectroscopic methods and related resolution-enhancing techniques
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. The implication is that EV-limit ensembles for proteins are likely to be close facsimiles of conformational ensembles in high concentrations of chemical denaturants
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. Taken together, these data suggest that there were structural changes, which partially solvent-exposed the indole moiety and/or tyrosine residues, and modified the ellipticity at 222 nm (41,42)
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. In the solutions containing 100 mM NaCl and 10 mM acetate buffer alone, this interaction becomes increasingly attractive at short range
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. In the case here, the effect of the weak attraction between the BLG particles is an increase in η^sub r,0^ at a fixed φ
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. The existing morphological studies on insulin aggregation stress the hierarchical intertwisting of protofilaments (Jimenez et al
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. Since the MG conformation is more flexible than a fully folded polypeptide, this could lead to better packing at the dimer interface, leading to more stable dimers
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. Do harshly denaturing environments such as 8 M urea or 6 M GdnCl mimic perfect solvents? In previous work, we developed a fast and accurate way to generate thermal, self-avoiding distributions for proteins with atomistic detail (48)
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. 2 A): this transition was more clearly observed when the protein was excited at 295 nm than when excited at 280 nm, suggesting that it was due to Trp^sup 461^
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. The endotherm at pH 7 (Fig
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. At the moment, however, we do not know whether these small changes in the structure are also responsible for the observed considerable specificity of the phosphotransfer reaction between EI and HPr among the different species and the differences in their cnthalpic variations in their binding reactions (45
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. Among a large variety of different methods, such as absorption-, circular dichroism spectroscopy, calorimetry, NMR, and light scattering techniques, in particular fluorescence spectroscopy is a powerful technique to monitor unfolding transitions and to characterize properties of the unfolded state (see for example (3,4))
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. Such work is ongoing in our laboratories, and data from misfolded TTR oligomers recorded from site-specific fluorescence labeling of cysteine mutants have provided size distributions with average rotation correlation times of ~ 150 ns (K
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