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. The molten globule state as a clue for understanding the folding and cooperativity of globular-protein structure
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. In the presence of even slower internal motions it becomes progressively difficult to distinguish overall tumbling and internal motion (22,32)
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. Fluorescence characterization of different protein conformersThe A-state of TTR binds the classic hydrophobic dye ANS (Lai et al
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. Although the blank fluorescence for DCVJ is low, DCVJ in the presence of unfolded TTR shows even lower fluorescence than the blank, which could indicate a small quenching effect through binding
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. Despite limited accuracy in the particle size determination and limited resolution, this system facilitates observation of lipoproteins, lipid-free and lipid-poor proteins on the same gel and is widely used for lipoprotein analysis (Davidson et al
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. As a result, disks containing larger proteins have lower unfavorable entropic contribution TΔS* to their kinetic stability ΔG* = ΔH*-TΔS*
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. [FIGURE 6, 7 & 8 OMITTED]Similar to the ANCOVA results for abso-lute fecundity, there were no differences inslopes or elevations for relative fecundity forprefertilization females for the years 1996-98 (Table 4)
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. Additional funding was provided through Hatfield Marine Science Center scholarships: the 1996 Barbara Schwantes Memorial Fellowship; the 1997 Mamie L
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. Monoclonal antibodies were developed according to the methods of Kohler and Milstcin (1975), Garfre and Milstein (1981), and Hiroishi et al
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. Thus, within the framework of these simulations and the two-state model, we can estimate the forward rate constant (k^sub 1^) for the extended-undocked to quasi-docked reaction to be in the 10 × 10^sup 3^- 41 × 10^sup 3^ s^sup -1^ range and the reverse rate constant (k^sub -1^) in the 3
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. First, the owl may not have ingested all of the bones of some of its prey
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. A 96 well plate was used and 40 µl of both the standards and samples were pipetted into each well; 250 µl of DMB solution was added to each and the absorbance at 595 nm was determined using a microplate reader (Bio-Rad Ltd, Hemel Hempstead, UK)
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. * According to NCPG, the following signs indicate that a teen might be developing a gambling habit: spending all of their allowance or paycheck on gambling; borrowing money to gamble; selling possessions to gamble; gambling for a longer duration than planned; talking about gambling as a way to make money; making repeated unsuccessful attempts to stop gambling
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. Investigation of the properties of these partially folded states using probes such as ANS or bis-ANS (which bind to exposed hydrophobic domains in proteins) was not possible due to the significant overlap between the emission spectra of these probes when bound to proteins and the excitation spectrum of the prosthetic group, FAD (data not shown)
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.[beta]0+[beta]1FL'where P(Y=1|FL) = probability of female black rockfish being mature at size FL; and[beta
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