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. She turned to Sudhoff again to secure loans for a two-story townhouse in Baltimore and an investment home in Hampton, Virginia
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. 6, containing 150 mM NaCl, at 23°C
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. 1, are turned off, we obtain Flory's freely rotating chain model (43), albeit with a constraint that the peptide units are all in a trans configuration
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. Conversely, at acidic pH, the percentage of β-turns appearing at 1668 cm^sup -1^ was decreased, and the percentages of helical structure, loops, and disordered structures (bands at 1648 and 1657 cm^sup -1^, which could not be independently assigned at pH 3) were higher (50
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. Sugar transport by the bacterial phosphotransferase system
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. The Φ angles, recalculated with the smaller anisotropy, were essentially within the error margins of the earlier estimates
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. 1 (10 mM buffer) and NaCl concentrations of 0 mM, 100 mM, and 1 M
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. For small globular proteins, such as BLG, the volume of this layer is comparable to the volume of the dry protein
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. It is recognized that the binding of the part of monocations to DNA can be specific in the minor or major grooves of B-form DNA (for review, see Hud and Polak 2001; McFail-Isom et al
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. RESULTSMeasurement of protein-membrane associations under pressurePrevious studies of model membranes underpressure suggest that the primary effect of pressure is in the compressible hydrocarbon region, and the application of pressure increases the gel to liquid crystal phase transition by a constant that depends primarily on the lipid chains (Li and Kay, 1977)
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. , 1999), but which do not bear an interpretation in terms of native structure (Fersht, 1999)
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. (1989) reported that striped bass eggs stripped from wild fish increase in quality with maternal age due to increased amounts of proteins and lipids, although relative concentrations remain unchanged
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. Using these estimates, along with the fork length at 50% maturity, we estimated the age at 50% maturity for female black rockfish to be 7
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. These results are consistent with those obtained in a study conducted by Damush et al
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. "Half of the people I see don't even know what kind of loan they have," says Sudhoff
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. He resides in Cheshire, Conn
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. The curves from the diluted solution (data not shown) were similar to those presented in Figs
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. Conversely, P(x) is maximal for small x if one assumes a Flory-style random coil model with uncorrelated fluctuations and uniform (mean-field) chain swelling
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.CONSEQUENCES OF DEATH AND REMOVALBurying the BodiesThe most immediate consequence of efferocytosis is the physical removal of apoptotic cells before membrane permeability sets in, thus preventing the release of potentially toxic intracellular contents
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. A similar behavior, which is typical for most proteins (20), is also observed for TAKA, BSUA, and BAA (see Table 2)
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. Protein interactions and crystallization
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. Therefore, the widely accepted correlation between cation binding and compaction potential is not an absolute
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. Although the fluorescence lifetime of ANS is not in the range to enable accurate determination of the rotational correlation time of a large oligomer, ANS anisotropy decay can be used as a measure of aggregation both through point measurements and in a kinetic assay showing limiting anisotropy baselines
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. The +4°C shift, the reduced height, and the increased width (decreased cooperativity) of this peak as compared to the sharp transition in DMPC vesicles at 24°C (Chapman, 1975) are characteristic of lipoprotein disks that have smaller co-operative lipid domains than vesicles
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.Heating of apoA-2:DMPC disks from 5 to 70°C, i
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. This can negatively affect mix stability and fat agglomeration in the freezer
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. Because the tip approaching the surface induces a shift of AFM cantilevers' resonance frequency, the drive frequency, which has to be adjusted before engagement of the oscillating probe's first surface contact, is reduced until the vibrational amplitude reaches 95% of the maximum value
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. The driving force transforming destabilized protein molecules to protofibrils, and protofilaments to fibrils, arises from hydrophobia and electrostatic interactions
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. Although considerable evidence suggests that only partial destabilization of insulin is the prerequisite for its aggregation (Ahmad et al
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. Samples were either frozen or fixed in 5% formalin to seawater solution
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. and plotted in the form of a histogram
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