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. Using these procedures, the energetics and pathways of folding, as well as the existence of transient intermediate states between unfolded and fully folded polypeptides, have been characterized for a number of proteins (for a collection of reviews, see Creighton, 1992; Pain, 1994)
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. The maximum wetting forces and corresponding meniscus heights are estimated through the finite-element modeling by varying the parameters such as substrate perimeters, immersion depths, and results, as shown in Figs
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. For the 54% w/w solution, G' crosses G'' at ~2 Hz, a transition to an elastic solidlike response
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. Calculations (21) show that if a longer ranged attraction is added on to the AHS model, although the percolation line does not move, the effective stickiness varies such that the liquid-liquid coexistence line is no longer below the percolation line, a potential explanation for the absence of particle gelation
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. Therefore, the following studies were conducted assuming that properties of the membrane surface are largely unchanged in the 1-2000 bar range
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. semifasciata, and most of what is known is based on limited observations during underwater visual censuses on shallow reefs where adults concentrate (Gonzalez, 1998)
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. Far-ultraviolet (UV) circular dichroism (CD) measurements were made in a 0
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. The protein concentration used for the studies is 0
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. The unite probability of linding large cavities within the interior of denatured proteins emphasizes two points: First, the volume occupied by a chain is filled inefficiently when compared to either a folded protein or a freely rotating chain
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. Experiments were carried out in the absence and in the presence of the effectors (at final concentrations of 2
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. The biological relevance of the partially folded species at acidic pH is unknown, and we do not know whether the residual secondary structure detected by CD and FTIR (Table 2) was native-like
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. The delay between the 180° pulses (δ) in the CPMG was set to either 450 µs or 3
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.ANS, Bis-ANS, DCVJ, and ThT fluorescence as probes of different TTR conformersFluorescence spectra of the different molecular dyes in the presence of native and misfolded aggregated states of TTR are displayed in Fig
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. Cratic entropy for dissociation of a single polypeptide is lower than that of its fragments; similarly, it is lower for larger proteins
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