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. The unfolding of this β-sheet allows the water molecules to penetrate into the interior of the catalytic domain, which results in the transformation of some Class I fluorophores to Class II and a further inactivation of the enzyme
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. 4, and the molarity of guanidinium hydrochloride was determined by refractive index as described by Pace (20)
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. Dynamic light-scattering studies The dynamic light scattering measurements were done using a DynaPro-MS800 dynamic light scattering instrument (Protein Solutions, Lakewood, NJ)
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. R^sub e^, and internal distances scale with sequence separation according to universal power laws
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. If, for example, a tryptophan residue becomes more accessible to the solvent, which quite often is the case for unfolded states, the emission intensity decreases
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. In the cytoplasm, the drug signal was superimposed with the natural triglyceride peaks
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. In solution, this helix is disrupted in the middle (2), which allows N- and C-terminal domains of both apo-CaM and Ca^sup 2+^/CaM to tumble almost independently of each other (3-5)
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. This assumption has been used to explain the dependence of DNA-monocation binding
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. Further work seems here necessary, and fluorescence probes exhibiting longer decay time is highly desirable for direct determination of the size distribution of the oligomer assemblies and protofibrils
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. , ≤1 kbar), we find that the volume change of PLCβ-Gβγ association is reduced
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. Repetitive T-jumps with DMPC complexes containing apoA-1, apoC-1, or apoA-2 show that the protein unfolding in the first jump is slower than that in consecutive jumps (Fig
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. On average, CD4 shows an intraresidue entropy decrease of -0
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. The evolution of phenotypic variance with iteroparity
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