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. The average volume occupied by the chain will be filled inefficiently, and cavities of all sizes l > ls should be found readily within the interior of a denatured protein
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. The stable intermolecular hydrogen-bond network (166 and 130 atoms for CD4 and gp120, respectively; for details see Table 1)
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. There are significant differences in the thermal stabilities of the two forms
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. As a result, for each sequence, we generated an ensemble consisting of 10^sup 4^ uncorrelated conformations
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. The fluorescence emission of the folded state is already rather blue shifted (λ^sub max^ = 327 nm), but also rather large λ^sub max^-values (341-344 nm) for the unfolded states contribute significantly to the large red shift
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. The sample was thereafter stained using 2% uranyl acetate for 20 s, and the grids were dried overnight
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.3 kJ K^sup -1^ mol^sup -1^ and ΔS^sup 2nd,b-f^^sub gp120,all^ = 0
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. If instead, subpopulations interconvert rapidly on the burst-duration timescale, a single averaged peak will be observed, with increased broadening as the interconversion rate approaches this timescale
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