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In this research, the sequential events in ACYI thermal unfolding were investigated by a combination of spectroscopic methods and the related resolution-enhancing techniques
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Samples were incubated at 25°C for 10 min
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A receptor for phosphatidylserine-specific clearance of apoptotic cells
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The unfolding was triggered at t = 0 by a temperature-jump from 25°C to 75-95°C and was monitored by CD at 222 nm (Fig
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A challenge faced by all these models is that the most successful search for inherent features of tertiary structure that correlate with folding rates has found that the topological measure of "contact order" is far more closely related than, for example, molecular weight itself in the case of "two-state" folders (Plaxco et al
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Ribozyme constructs missing the substrate strand were prepared in annealing buffer (missing NaCl and supplemented with 12 mM MgCl^sub 2^) at a concentration of 2 µM of each strand and annealed by heating to 70°C for 2 min and cooling to ambient temperature
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. Several functions for MG intermediates have been proposed, including binding to chaperones for correct folding (Bochkareva et al
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. Partitioning the configurational space in this way, and by providing an attractive "gutter", relying on local forces alone, to connect one diffusive subspace to the next, creates the advantageous funnel landscape when the high-dimensional structure is projected into a one- or two-dimensional space
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.Two sets of atoms were used in the superposition of molecular configurations:1
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