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. In native proteins containing both tyrosine and tryptophan residues, tyrosine emission is not commonly observed due to efficient energy transfer from tyrosine to neighboring tryptophan residues (Lakowicz, 1983)
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. 1% of the residues in the most favorable regions and 10
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. In this pH range, the minimum at 208 nm disappeared, whereas that at 222 nm was still present
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. Table 2 shows the required gradient field strengths for the respective voxel size and rf bandwidth
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. Protein unfolding was triggered by an increase in guanidinium hydrochloride (Gdm HCl) concentration from 0 M to 1-6 M
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. This exemplifies that the correct criterion for thermodynamic reversibility is the absence of scan rate effects and hysteresis rather than the repeatability of a transition
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. This is both natural and necessary, since data from kinetic experiments do just the same, but there is a danger in overlooking aspects of folding that rely essentially on the presence of many degrees of freedom
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. Since the diffusion is always maintained in some low-dimensional subspace of the full folding space, τ ~ R^sup 2^/D for each subspace, so that τ^sub fold^ ≈ d^sup 2^R^sup 2^/D rather than the exponentially larger (R^sup 2^/R^sup 2^^sub N^)^sup d^
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. The spectra were collected at a scan speed of 50 nm min^sup -1^
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. RESULTS In this work, we focus on two-state proteins because the hypothesis is that only two well-defined macrostates-native and highly denatured states-are accessible to these systems (67-70)
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. Transcriptional suppression of interleukin-12 gene expression following phagocytosis of apoptotic cells
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. The obtained peak maxima around 336-338 nm indicate rather small red shifts (~2-3 nm) with respect to the native state (see also Fitter and Haber-Pohlmeier (14))
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.TheoryThe equations for the relaxation of a backbone amide ^sup 15^N spin in a protein as well as their interpretation in terms of the model-free approach of Lipari and Szabo (25) are well described in the literature (see, e
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. The discrepancy at the lower concentrations (mass fraction ~7-16% w/w) could be decreased if the assumed molecular weight (MW) were reduced from the dimer MW to the monomer MW
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.01 mM to 1 M Mg^sup 2+^
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