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and those made by us, we may conclude that rSBA and gSBA are similar in all other respects (secondary and tertiary structure, size, and function) except for glycosylation (as shown by the difference in the matrix-assisted laser desorption ionization mass spectra in Supplementary Material, Fig government grants for home loan .
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1 B) at wavelengths ~208 nm government grants for home loan .
The other major change in a fluorescence signal upon conformational changes of the protein is related to solvent relaxation, which determines the magnitude of the stokes shift government grants for home loan .
Because the spectrum reflects the hydrophobicity of the binding site, shifting to the blue in less polar environments (30,31), the above correlation suggests that extensive binding of ANS is associated with the exposure of hydrophobic regions of the macromolecule that are generally buried and inaccessible to the probe government grants for home loan .
How soft is a protein? A protein dynamics force constant measured by neutron scattering government grants for home loan .
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This timescale corresponds well with the expected motions of the N- and C-terminal CaM domains government grants for home loan .
9, or increased above 10, the presence of a second slower diffusive process in the autocorrelation function was observed, which was attributed to a very limited aggregation/association of BLG (34) government grants for home loan .
It was reported that compact DNA in PEG solution demonstrates specific shape of circular dichroism spectra (the so called Ψ-spectra) assigned to severe distortion of typical B-form shape of DNA helix (Evdokimov et al government grants for home loan .
In addition, CD spectra of DMPC-bound apoA-2 were recorded from solutions of 5-600 µg/ml protein concentration placed in 10-0 government grants for home loan .
6 and 7 B) differs from the stability ranking apoA-2 > apoA-1 that is inferred for plasma spherical HDL and is attributed to deeper penetration of the more hydrophobic apoA-2 α-helices in the lipid milieu (Brasseur et al government grants for home loan .
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FRET histograms were fit with multiple Gaussian functions using Origin (OriginLab Corp government grants for home loan .
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