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. Thus, the value for K^sub SV^ will depend on the particular average intrinsic fluorescence lifetime of the protein, and may be higher than the value for free Trp
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. Further, the model was evaluated with PROCHECK (37)
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. 1 A); however, there were changes in the secondary structure, as reported by CD (Fig
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. Circular dichroism (CD) and light-scattering melting curves show hysteresis and scan rate dependence, indicating thermodynamically irreversible transition with high activation energy E^sub a^
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. However, the molar ellipticity at 222 nm at basic pH was larger (in absolute value) than that at pH 7
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. The charged thiazol moiety of ThT likely serves as an intrinsic intramolecular quencher of the ThT molecule
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