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Although the thermal unfolding of many proteins can successfully be illustrated by a two-state model, step-wise transitions have been observed in several proteins regardless of whether the process was reversible or irreversible (30,38, 39,49-54)
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Second, the magnitude of fluctuations in interresidue distances increases with increasing sequence separation
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Three probes-ANS, DCVJ, and ThT-show increased fluorescence throughout the aggregation reaction, whereas Bis-ANS shows fluorescence decay (Fig
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In the same way, if denaturant concentration affects the stability of the low-dimensional search spaces, or gutters, that are principally responsible for controlling the folding rate, then these experiments will also carry signatures of the search-structure
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The system was dissected into various subsets of atoms to evaluate the entropic contributions of different functional elements
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Clearly, these simulations do not describe the data as well
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Ratiometric single-molecule studies of freely diffusing biomolecules
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Sections were picked up onto copper grids, stained with 2% uranyl acetate and lead citrate and viewed on a Philips CM12 Electron Microscope at 80 kV
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31)Mental Component ofHRQOL 40
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Each player was given HK$20 (approximately US$2
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