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5 and 3 M, and that a predissociated state with an expanded hydrodynamic radius compared to the native dimer is stabilized at 2
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maxima GR by GdnHCl has been proposed (Rendon et al
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To verity the results obtained in Fig
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The recent work of Riisgen et al
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INTRODUCTION NMR allows detailed insights into molecular properties of liquids or solids, including, for instance, chemical structures and dynamics (1)
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The CaM:SEF2-1mp complex is found to be highly flexible with internal motions on the picosecond, nanosecond, and microsecond timescales
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TheoryThe equations for the relaxation of a backbone amide ^sup 15^N spin in a protein as well as their interpretation in terms of the model-free approach of Lipari and Szabo (25) are well described in the literature (see, e
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As expected from Fig
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5, C and D) and shorter fluorescence lifetimes (data not shown) reveal Trp to ANS and Bis-ANS fluorescence resonance energy transfer both in the native and misfolded states, indicating that the Tips and the probes are in close proximity (Conrad and Brand, 1968)
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After the buffer baseline subtraction, the data were normalized to protein concentration and expressed as molar residue duplicity, [Θ], No changes in [Θ] were detected with changes in the protein concentration in the range explored (5-600 µg/ml)
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The increased temperature also appears to accelerate the elongation stage
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Originally conceived as a kinetic theory in which a nucleus of native structure corresponds to the transition-state for folding, the picture now also encompasses the hierarchical folding routes of the diffusion-collision model
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