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. We can speculate, however, based on the modeled three-dimensional structure of the EIC^sup sc^
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. Circular dichroism CD spectra in the far-UV region (200-280 nm) were recorded on a Jasco J-810 equipped with peltier thermostating cuvette holder under constant nitrogen flow
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. ANS, Bis-ANS, DCVJ, and ThT fluorescence as probes of different TTR conformersFluorescence spectra of the different molecular dyes in the presence of native and misfolded aggregated states of TTR are displayed in Fig
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. Similar to the CD data, the light-scattering heating curves of the apoA-2:DMPC disks shift to lower temperatures at slower scan rates, indicating high activation energy for the disk fusion
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. Furthermore, the amplitude of the light-scattering changes in apoA-1:DMPC complexes is significantly smaller than that in apoA-2 complexes, suggesting that apoA-1 inhibits disk fusion more effectively than apoA-2
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. AN EXAMPLE: THREE-HELIX PROTEINSThe three-helix example illustrates our general conclusion that searches within diffusional subspaces in protein folding may be accelerated by local, but not necessarily native, interactions between sections of partially structured chain
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. This type of structure has been investigated theoretically (Wagner and Kiefhaber, 1999), and evidence for its rather general emergence has arisen experimentally very recently (Pappenberger et al, 2000; Sanchez and Kiefhaber, 2003)
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. Lastly, kinetic information about equilibrium interconversion of subpopulations (on microsecond to millisecond timescales) is uniquely available from spFRET data
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