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. For the sample with no added NaCl, the divergence occurs at a mass fraction of ~52% w/w, increasing to 56% w/w at 1M NaCl
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. Moreover, all Phe^sup 43^ side-chain conformations in the complex are contained within the ensemble of conformations of the free form
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. The histogram of interresidue contacts can be plotted as a contact density map and the results are shown in the top row of Fig
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. 12 support the conclusion that EV limit ensembles are topologically equivalent
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. Given the equivalence between the EV limit and highly denatured states, our ability to simulate conformational ensembles in the EV limit, with full atomic detail, has direct bearing on the development of an accurate physical picture of conformations accessible to denatured proteins
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. This feature is shared with Gaussian chains (39), although clear differences exist in the magnitude of and correlation between conformational fluctuations
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. (33) The second pathway includes ced-1, ced-6, and ced-7 (representing the mammalian homologues LRP, GULP, and ABC-A1 or ABC-A7, respectively)
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. In these exploratory experiments conducted with copper-free azurin from Pseudomonas aeruginosa as a model protein system, it was shown that upon freezing of the protein solution the fluorescence of ANS is enhanced severalfold and becomes blue shifted relative to free ANS
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. The remaining native fraction after 15 freeze-thawing cycles is listed in Table 1 where it is compared to the respective values of n and average dissociation constant K^sub d^
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. Additionally, the quasi-docked species appears to form very rapidly (see below), suggesting that this species could act as a kinetic intermediate in the docking pathway of the natural ribozyme
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