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. MATERIALS AND METHODS Materials Sodium dodecyl sulfate (SDS) was purchased from Sigma (St
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. The final enzyme concentration for the activity assay was 2 µM (~0
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. The lateral association is seen in the corresponding AFM images after 30 min at 60°C, but already after -20 min at 70°C
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. Gray Davis backed the measure
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. coli GR by NADPH has been previously described (Arscott et al
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. As noted by Chan and Dill in their influential review (37), theories drawn from the polymer physics literature (38-44) are well-suited to describe heterogeneous conformational ensembles such as those of denatured slates
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. If one were to zoom into the contact density map of a long protein such as titin one reproduces the contact density map for a shorter protein such as uhiquitin or peripheral subunit binding domain (PSBD)
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. Protein was extensively dialyzed against 2 L of the working buffer (5 mM HEPES buffer containing 10 mM NaCl and 2 mM β-mercaptoethanol, pH 7) at 278 K
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. From the often drastic shortening of the phosphorescence lifetime of Tip residues buried within the rigid core of these macromolecules, it was deduced that their internal flexibility is greatly enhanced in ice, a response to freezing similar to that observed when the polypeptide loses much of its tertiary structure as in the formation of molten globule states
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.Data analysisAll spectra were analyzed using SYBYL software (TRIPOS)
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. In the experimental situation, although the protein particle is expected to be monodisperse, in practice a limited aggregation is often observed
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. For a random packing of noninteracting monodisperse hard spheres, these structures may form at volume fractions in the vicinity of 0
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. DCVJ, a molecular rotor, was employed for the first time for studies of an amyloidogenic process and is shown useful for detection of the early steps of the oligomerization process
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. DCVJ is intrinsically quenched and belongs to a class of fluorescent probes known as molecular rotors
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. None of the eggs fixed just after collection were morphologically identifiable
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. There was also no significant difference between the advice groups in either number of losses, F(1, 14) = 3
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