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. The PK^sub a^, leading to the acidic species could be determined from the ANSbinding and intrinsic fluorescence measurements, suggesting that one aspartic and/or glutamic acid must be responsible for this transition (43)
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. In particular the wavelength of the tryptophan fluorescence emission peak (λ^sub max^) is a valuable parameter to characterize properties of the unfolded state
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. Involvement of the hydrophobic patch of azurin in the electron-transfer reactions with cytochrome-C551 and nitrite reductase
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. Modifications induced upon oligomerization in high-frequency, small amplitude/localized modes, which do lie within the sensitivity of our experimental window, might be rather small
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