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. Our results show that the secondary structure of EI^sup sc^ was not substantially altered, as suggested by FTIR experiments, but quenching experiments and proteolysis patterns suggest that there were small conformational changes upon binding of PEP; in addition, the thermal stability of EI^sup sc^ was slightly modified when any effector was added
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. To isolate the contribution of the helical junctions to tertiary folding, we also studied the corresponding ribozyme constructs without the loop on arm A (SL 2WJ, 3WJ, and 4WJ, Fig
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