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. The plates were developed in chloroform/methanol/2
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. Repetitive jumps to other temperatures yielded similar results
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. Another interesting object visible in Fig
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. Samples were either frozen or fixed in 5% formalin to seawater solution
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. This analysis showed a single protein band in the GR preparations used, indicating that the protein was essentially pure
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.8-ml cell (Setaram Scientific and Industrial Equipment, Caluire, France)
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. Two-dimensional infrared correlation (2D IR) analysis was carried out using SDIAPP software developed in-house (27) according to the generalized two-dimensional (2D) correlation algorithm (29)
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. To evaluate the effect of the protein concentration on the fluorescence spectra, a diluted sample solution with a final concentration of 0
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. Further, the unfolding pathways in the two are widely different
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. Is protein folding hierarchic? II
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.Compounds that alter the Rac-1/RhoA balance, by either increasing the level of active Rat-1 (preferable) or decreasing the levels and/or activity of RhoA/ Rho kinase, would be potential candidates for use in therapy
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. To aid comparing the ANS binding propensity among the various proteins Table 2 lists the minimum number of binding sites (n) as well as the ratio n/Mw, normalization by the molecular weight taking into account that larger macromolecules, such as oligomers, have a greater probability to bind ANS simply for their larger mass
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.To confirm that our labeled ribozyme constructs are active in cleavage, we performed activity assays using a cleavable all-RNA substrate strand, as described in the Materials and Methods section
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. The 21st edition continues as a practice-oriented text with substantial scientific depth
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. Upon request, training sessions are available for individual pharmacists or groups of pharmacists
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