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Classical properties of the MG state include increased molecular volume caused by an increase in internal water content (Kuwajima, 1989), preservation of nativelike secondary structure (Nozaka et al
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ACYI has been successfully applied in the industry to produce L-amino acids (14,15)
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Infrared spectra of amide groups in α-helical proteins: evidence for hydrogen bonding between helices and water
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For all sequence separations that go beyond ~7 residues, ensemble-averaged internal distances show the same power law behavior as R^sub g^ and R^sub e^
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Although we observe a qualitative proportionality between smaller red shifts and a significant aggregation of thermal unfolded states (at least for BAA, BLA, and BSUA), we can not exclude other and maybe additional mechanisms responsible for smaller red shifts during thermal unfolding as compared to those obtained with GndHCl-induced unfolding
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3 B emphasizes that the value both of n and of the apparent dissociation constant are considerably affected by temperature
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In conclusion, residues with fast exchange can be identified from the combination of R^sub ex^ and ΔR^sub ex^ using either the fast exchange equations or the general fast/slow exchange equations derived by Ishima and Torchia (34)
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, 1998) and for transthyretin (TTR) (Sousa et al
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