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. But the complexity and sophistication of the efforts that have been needed to reach this resolution show how, at times, a simple question can open up penetrating new leads into the labyrinth of evolutionary theory
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. The latter is a model for a soft, Gaussian coil with large-scale, albeit uncorrelated
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. Results for ubiquitin modeled as a freely rotating chain are shown in Fig
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. Murine macrophages produce secretory leukocyte protease inhibitor during clearance of apoptotic cells: implications for resolution of the inflammatory response
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. Anilinonaphtalene sulfonate as a probe of membrane composition and function
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. , limited to the active site), concern low-frequency collective modes below the sensitivity of the technique used and/or present only on a longer timescale (> 1 ns), and not directly related to fast thermal fluctuations
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. Figure 5c reveals that WS flux results in better wettability among the three fluxes on the Ni substrate
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. Here, the average (τ^sup o^^sub m^)^sub app^ values for the α-helices are given in parentheses and the error on Φ was based on a uniform error in (τ^sup o^^sub m^) of 1
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. Other assumptions will change the relative size of the rollover effect, but not remove its qualitative presence
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. The configurational entropy analysis in terms of combined trajectories presented here shall potentially provide accurate estimations of thermodynamic properties of biomolecules given sufficient sampling of conformational space
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. and plotted in the form of a histogram
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. In addition, the conformer populations are obtained directly from the areas under each peak in the spFRET histograms, rather than being treated as adjustable parameters in the trFRET analysis
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. Zaccai*||* Laboratoire de Biophysique Moléculaire, Institut de Biologie Structurale, Grenoble, France; [dagger] Centre de Recherches du Service de Santé des Armées, Unité d'Enzymologie, La Tronche, France; [double dagger] Institut de Pharmacologie et Biologie Structurale, Toulouse, France; § Division of Biochemistry, Walter Reed Army Institute of Research, Silver Spring, Maryland; ¶ Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel; and || Institut Laue-Langevin, Grenoble, FranceSubmitted February 9, 2005, and accepted for publication June 30, 2005
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