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. In EI^sup sc^ at low pH, the fluorescence intensity of ANS was largely enhanced, and the maximum wavelength of the fluorescence spectrum was 480 nm (Fig
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. The fluorescent dyes 1-anilinonaphthalene-8-sulfonate (ANS), 4-4-bis-1-phenylamino-8-naphthalene sulfonate (Bis-ANS), 4-(dicyanovinyl)-julolidine (DCVJ), and thioflavin T (ThT) were employed in both static and kinetic assays to characterize these oligomeric and protofibrillar states using both steady-state and time-resolved fluorescence techniques
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. The plates were developed in chloroform/methanol/2
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. Similar unfolding below 25°C was detected in the earlier studies of human lipid-free apoA-2 at much higher protein concentration (>0
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