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The fluorescence emission of native GR is quite blue-shifted ([lambda]^sub max^ at 317 nm), indicating that, on average, the four tryptophan residues in each GR subunit are well-protected from exposure to the aqueous medium in the folded protein gambling game roulette .
The resultant data are used to compute P^sub oa^(r), which is defined as the probability of linding a cavity of radius a at a distance r from the center of mass in the ensemble gambling game roulette .
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Since the diameter of this gorge, at its narrowest point, is smaller than the cross-section of the quaternary group of ACh, substantial breathing motions of the whole AChE catalytic subunit are necessary to allow the substrate to penetrate to the active site gambling game roulette .
This corresponds to ΔH^sup #^ values of 8-18 kcal/ mol, when employing a two-state exchange model, Eq gambling game roulette .
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The C^sub p^(T) data indicate a gradual reduction in the vesicle population and a concomitant increase in the disks population during incubation (Fig gambling game roulette .
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As a result, the antibodies were reactive with all the P gambling game roulette .
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