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Sometimes I can strike up a conversation and carry out the whole airport check-in in such a casual way that my consort fulfills his role unconsciously
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. It has been proposed that the catalytic domain contains a β-sheet sandwiched between α-helices and a second β-sheet located on the surface, whereas the dimerization domain consists of a β-sheet flanked on one side by two α-helices (17)
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. This modification involves a number of enzymes and substrates and implements numerous processing steps
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. Proteins in high concentrations of denaturants, such as 8 M urea or 6 M GdnCl, behave like chains in good solvents (3)
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. This energy difference is used with the Metropolis criterion (61) to accept or reject the proposed move
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. As the associated shear stress was [much less than]50 kPa, we ascribe this behavior to being equivalent to the η^sub 0^ of hard sphere colloidal suspensions
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. 75 ml g^sup -1^ was then used to obtain the mass of water in the hydrodynamic domain of the protein, and from this the volume fraction of protein at known mass fractions was estimated
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. In the case here, the effect of the weak attraction between the BLG particles is an increase in η^sub r,0^ at a fixed φ
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. The DSC data recorded of apoA-1- and apoA-2-containing disks upon heating at 90 K/h show that the unfolding/ dissociation of apoA-1 is centered at 91°C, ~7°C higher than the apoA-2 transition (Fig
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. Phase diagram analysis of IR and fluorescence data The phase diagram analysis, which is a sensitive tool to detect folding intermediates, was carried out as described previously (35)
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