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8), 150 mM sodium chloride, 15% glycerol, 1 mM Ca^sup 2+^/Mn^sup 2+^, and 200 mM lactose) for 1 h with mild shaking in cold
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Beyond this length scale, all internal distances scale with sequence separation in accordance with the standard power law for proteins in a good solvent
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As a consequence, the EV limit is characterized by hierarchical contacts whereby the distribution of distances between near-neighbor residues is narrow and peaked around smaller values
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2 should not be considered a binding curve, it may still be instructive to estimate an apparent average affinity of ANS for the four azurin species
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02 that were obtained following Meylan and Howard (28)
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In the following, this sample is denoted as DmAChE-H2O
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Analysis of a 10-ns molecular dynamics simulation of mouse acetylcholinesterase
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For k^sup -1^^sub ex^200 µs
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47 ml g^sup -1^, obtained from the R^sub h^ of the BLG monomer in dilute solution, leads to values of φ for the more concentrated solutions which are above the expected φ^sub max^ or, using the reverse of the above conversion, a mass fraction of 43
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Thus, pressure methods allow for the estimation in the volume associated with the Gα^sub s^(GDP) deactivation of the PLCβ-Gβγ complex even though the change in interaction energy between PLCb and Gβγ caused by Gα^sub s^(GDP) could not be detected by spectroscopic atmospheric pressure measurements
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Effect of increased lipid packing on the surface charge of micelles and membranes
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Thus, the initial state in the first jump was intact disks, and in the second jump it was a mixture of protein-containing vesicles and lipid-poor protein
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Alternatively, our picture suggests ways of increasing folding times greatly by selective mutations that retain topology and stability of the native state, but destabilize one or more of the on-pathway diffusional subspaces so that intermediate searches are required in d > 3
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. We deeply thank both reviewers for their criticisms, ideas, and suggestions
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