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. It is tempting to suggest, based on our results, that the species populated at high pH is also a molten-globule-like species, although it did not bind ANS (Fig
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. In total, 116 residues were analyzed using the PINATA method (22), which is particularly suited for proteins that exhibit extensive nanosecond-timescale internal motions
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. The exchange broadening must then be due to conformational exchange between different conformations of bound CaM
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. INTRODUCTIONDNA compaction in living cells is a process of extraordinary biological significance
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. We have previously shown that Gα(GDP)-subunits cause de-activation of PLCβ-Gβγ without significantly affecting their association (Runnels and Scarlata, 1998)
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. The T-jump data to 95°C are well approximated by a single exponential, yet two exponents are required to fit the data at 75-90°C, suggesting two kinetic phases
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. Two opercular spines were also present in all specimens studied
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. Second, the accessibility of the aromatic residues and structural changes occurring upon binding of the effectors to EI^sup SC^ was tested by acrylamide quenching experiments and trypsin digestion experiments
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. For water suppression, three successive sinc3 pulses of 19 ms were applied along with spoiling gradients of 6 ms and 120 mT/m
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. Both the analytical expressions for tumbling times of cylinder symmetric objects as well as bead model hydrodynamics incorporated in the DASHA software (24) were used (see Supplementary Materials for more details)
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. Further work seems here necessary, and fluorescence probes exhibiting longer decay time is highly desirable for direct determination of the size distribution of the oligomer assemblies and protofibrils
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. but not LpA-2, provide substrates for LCAT, formation of LpA-1/A-2 may be facilitated by the relative ease of spontaneous LpA-2 fusion
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.DISCUSSIONSufficient conformational sampling is a prerequisite for a reliable estimation of the conformational entropy
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