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. Thus the intrinsic Trp fluorescence was used to investigate the changes of the tertiary structure of ACYI during thermal denaturation
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. Protein stability in mixed solvents: A balance of contact interactions and excluded volume
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. (See the "Understanding Milk" sidebar
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. Although the fluorescence intensity signal exhibits this proportionality to the population of the microstates (i
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. Accordingly, the minimum number of bound ANS molecules (n) increases from 1
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. The true number is probably larger because on average protein sites exhibit ANS fluorescence yields much lower than 0
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. In considering data obtained by x-ray crystallography, Frauenfelder and Petsko (11) found that binding of oxygen was accompanied by a reduction in B-factors of residues close to the heme pocket in myoglobin
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. The authors thank Jacques Colletier for useful discussions on cholinesterase biochemistry as well as the IN16 instrumental team (Bernhard Frick, Thilo Seydel, and Matthias Elender) and the IN13-CRG team (Marc Bée, Francesca Natali, and Sebastien Vial) at the Institute Laue-Langevin, Grenoble, France
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. It was found that the lead-free Sn-2
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. Competitive electrostatic binding of charged ligands to polyelectrolytes: planar and cylindrical geometries
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. ANS, Bis-ANS, DCVJ, and ThT fluorescence as probes of different TTR conformersFluorescence spectra of the different molecular dyes in the presence of native and misfolded aggregated states of TTR are displayed in Fig
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. Abbreviations used: LpA-1, HDL containing apoA-1 but not apoA-2; LpA-2, HDL containing apoA-2 but not apoA-1; LpA-1/A-2
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. Such proteins would present a highly bimodal distribution of Φ-values, clustering closely to 0 and 1
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. VenerusAbstract--The Argentine sandperch Pseudopercis semifasciata (Pinguipedidae) sustains an important commercial and recreational fishery in the northern Patagonian gulfs of Argentina
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. For further simulation details, we refer to Hsu and Bonvin (2004)
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. 45 respectively) are due largely to the relatively poor correspondence between age and size (Fig
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. Birds represent the next largest group of vertebrate prey, averaging 6% of individual prey, or nearly 9% of biomass
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. The pellets cultured in DMEM/ ITS+/TGF-[beta]1 were larger and contained significantly greater amounts of DNA and glycosaminoglycans
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. Principles of Fluorescence Spectroscopy
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