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Some efferocytosis receptors appear to perform primarily a tethering function by holding the apoptotic cell and phagocyte close together (eg, CD14 or CD31), (19,21,22) while other receptors are primarily involved with transducing the engulfment signal (eg, LRP and PS receptors [PSRs]), (11,21,23,24) hence the so-called tether-and-tickle hypothesis (Fig 1, top, A) free internet gambling .
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This investigation aims to complement that initial study by addressing the following issues: i), establish whether the perturbation of protein structure reported for azurin is general to other proteins; ii), inquire on potential correlations between the susceptibility to the ice-induced perturbation and specific features of the macromolecule such as thermodynamic stability, internal flexibility, internal cavities/hydration, and covalent links between strands; and finally iii), test for a specific vulnerability of multimeric proteins to freeze damage that may be linked to the disruption of quaternary structure free internet gambling .
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