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We interpret these results such that although diminazene penetrates the cell membrane earlier than aceturate due to higher permeability, a large portion of it is strongly bound, and only a small portion of diminazene molecules weakly bound to nuclear macromolecules gives the initial signal
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The interaction is clearly different from the well-known categories of specific binding by lock-and-key or induced-fit binding, in which the complex in the bound state becomes essentially locked in one conformation
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