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. Asked if all these activities affected the outcome, Feeley told me simply, "I lost by only 121 votes
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. 9 ml/min^sup -1^, with intrinsic fluorescence detection at 315 nm (excitation at 280 nm)
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. However, when the concentration of GdnHCl in the sample was raised to 2
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. © 2007 by the Biophysical Society 0006-3495/07/01 /208/09 $2
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. Degrees of freedom Bond lengths and bond angles are fixed at equilibrium values taken from the work of Engh and Huber (59)
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. The resultant data are used to compute P^sub oa^(r), which is defined as the probability of linding a cavity of radius a at a distance r from the center of mass in the ensemble
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. In Protein Folding Handbook, Vol
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. Am J Respir Cell Mol Biol 2003; 29:S70-S76(15) Hu B, Sonstein J, Christensen PJ, et al
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. To contribute to the ongoing discussion and to investigate the relationship among molecular dynamics, activity, inhibitor-binding, and unfolding, we carried out incoherent elastic neutron scattering experiments on two members of the cholinesterase (ChE) family: Tetrameric human butyrylcholinesterase (HuBChE), both native and covalently inhibited by the organophosphate nerve agent, soman (18), and dimeric native Drosophila melanogaster acetylcholinesterase (DmAChE)
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. Figure 4a for the NC flux reveals that there is no significant increase in wetting force for Sn-Pb solder at the temperatures 255°C and 275°C, whereas the maximum wetting force increases slightly from 2
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. The model described above implies that activation of PLCβ2 by Gβγ-subunits occurs through one or more small interactions whose energies are within the experimental error of the measured affinities
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. The herein-discussed polymorphism of insulin amyloids, and the putative lateral assembling interactions between the seedlike forms and early nativelike molecules, are important-since both appear to correspond to in vivo cases of amyloid proliferation, and may contribute to a deeper understanding of the amyloidal-type of neurotoxicity, as well as the intra- and extracellular mechanisms of the propagation of amyloids
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. A peak close to zero FRET efficiency is always observed, and occurs in part due to photobleaching of the acceptor, as well as other processes that give rise to donor-only molecules (Deniz et al
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. Between 2 and 5 M GdnHCl, a significant drop in anisotropy was observed, indicating faster rotational mobility of tryptophan residues upon unfolding of GR
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. No changes in the elution volume of GR were observed when the sample contained 1 M GdnHCl
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. However, the low homology among the amino acid sequences of D- and L-aminoacylases available suggests that they may have different fold and catalytic mechanisms (2)
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. Although the thermal unfolding of many proteins can successfully be illustrated by a two-state model, step-wise transitions have been observed in several proteins regardless of whether the process was reversible or irreversible (30,38, 39,49-54)
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