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Thus, the value for K^sub SV^ will depend on the particular average intrinsic fluorescence lifetime of the protein, and may be higher than the value for free Trp
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Addition of 7 M GdnHCl completely abolished the CD signal at 222 nm, indicating extensive or complete unfolding of GR
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It has been studied in many aspects by using various methods
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Our models were in all cases (500 runs) unsuccessful, since the distance between both sites was very large to allow for the transference of the phosphate group (>10 [Angstrom])
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Higher protein concentrations lead to smaller red shifts upon thermal unfolding
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To look at the generality of the phenomenon, ANS binding experiments were carried out on seven new proteins, namely, monomers (bovine serum albumin (BSA), α-amylase, and β-galactosidase (β-gal)), a dimer (alcohol dehydrogenase from horse liver (LADH)), and tetramers (alcohol dehydrogenase from yeast (YADH), lactic dehydrogenase (LDH), and aldolase (ALD))
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We showed earlier that the pressureinduced circular amyloid of insulin consists of several loops of a thin and untwisting form, possibly a single protofilament (Jansen et al
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Such unspecific effects may contribute to morphological differences in any protein's amyloid samples induced at high temperature
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Thus we chose a dissolving temperature of 80°C
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5% said they had gambled at least once in a casino, compared to 59
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