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. Fully unfolded monomers were obtained at higher concentrations of GdnHCl
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. The aromatic chromophore 1-anilino-8-naphthalene sulfonate (ANS) is feebly fluorescent in water, but its spectrum is blue shifted and its intensity is dramatically increased in nonpolar solvents or when it binds to nonpolar sites of proteins (4,5; for a general review, see Slavik (6))
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. There are four families of Gα-subunits, and phospholipase C-β(PLCβ) is the main effector of the Gα^sub q^ family (Rebecchi and Pentyala, 2000; Rhee, 2001)
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. After this brief characterization of the gross stages of insulin amyloidogenesis, a more detailed analysis will be put forward
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. The configurational entropy analysis in terms of combined trajectories presented here shall potentially provide accurate estimations of thermodynamic properties of biomolecules given sufficient sampling of conformational space
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. DISCUSSIONSufficient conformational sampling is a prerequisite for a reliable estimation of the conformational entropy
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