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Spectra were averaged over three scans for each sample containing 3 µM GR in 30 mM HEPES, at pH 7
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Reconciling sequence-specific conformational preferences with generic behavior of denatured proteins
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The remarkable size may result from both the number of monomers involved, and the fact that insulin aggregation intermediates are rather bulky and swollen with water (Dzwolak et al
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The stabilizing interactions themselves may be either native or nonnative, in the latter case suggesting cases of tailored nonnative interactions
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In those 2 studies, however, the staining was entirely cytoplasmic, whereas the staining observed in our cases was mainly located on the cell membrane, with only some marginal cytoplasmic immunoreactivity
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