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The 2D IR plots were presented as contour maps produced by drawing the contour lines every 10% off from the maximum intensity of the whole correlation map
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This two-stage scheme is similar to that from the chemical unfolding/ refolding studies of ACYI (18,19), which also indicated that there existed a dimeric, aggregation-prone folding intermediate
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The precipitate was resuspended m 6 M guanidinium hydrochloride (in 10 mM Tris buffer (pH 7
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However, on increasing the denaturant concentration further, to 3
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Another noticeable feature is related to the fact that TAKA shows the largest red shift upon thermal unfolding
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A promising approach to elucidate the effect of aggregation in more detail and maybe on a quantitative level would be the use of encapsulation techniques
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Thermostability of irreversible unfolding alpha-amylases analyzed by unfolding kinetics
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In general, the wettability of Sn-2
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DNA compaction can be represented by a compaction curve as the dependence of the globule (compact DNA) fraction in solution on the concentration of the compaction agent, i
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, 2000), they are the last pre-amyloidal state with a native secondary fold
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The fiber's height at intersegment joints is only 3
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This in turn may assist in suppressing the pathway to aggregated states, or amyloid formation (Dobson, 2002)
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Since the docked and undocked peaks are well resolved, any interconversion between the two conformers must occur more slowly than the ~280-µs average burst duration of the single-molecule measurements (see below)
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(See Reproducible 2 for details
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