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This analysis showed a single protein band in the GR preparations used, indicating that the protein was essentially pure
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This choice is based on previous work (48)
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This different behavior in the thermograms among the members of a protein family is not new, and it has been reported, for instance, in the thermodynamic unfolding of dimeric histone-like proteins (53)
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1 for I7S, and >7
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(15) and references therein), MD simulations (16), and incoherent neutron scattering (17)
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These results suggest that activation of PLCβ by Gβγ is conferred by a small (i
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Preorganized secondary structure as an important determinant of fast protein folding
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LarvaeIchthyoplankton was sampled by using Bongo, Nackthai, and PairoVET nets
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This may reflect the large structural change and the crossing of the free and bound buildup curves (Fig
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5 mL Freund's complete adjuvant (Nacalai Tesque, Inc
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In addition, the occasional sub-optimalities in Basic (i
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.4 × 10^sup 9^ M^sup -1^ s^sup -1^ (Eftink and Ghiron, 1981)
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. In these studies, we observe that mostly the mannose residues are involved in the intersubunit interactions
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. In the presence of Gα^sub s^(GDP), the volume change associated with PLCβ-Gβγ interaction is reduced to 25 ± 1 ml/mol
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