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Folding/unfolding can be followed by measurements of biological activity and/or by a variety of spectroscopic methods
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The fluorescence emission of GR undergoes a marked red-shift between 1 and 3 M GdnHCl (with a transition midpoint at ~2
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" If there are no receptors, however, the neurons can be fully recruited and sequestered by other systems, making the brain as a whole work more efficiently
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the refolding efficiency after unfolding rSBA with GdnCl was much less, as monitored from fluorescence emission scans
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, ^sup 133^Cs and ^sup 31^P, exhibit chemical shift differences in different environments which can serve to discriminate between an intra- and extracellular location (19,20)
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1 B shows ^sup 1^H MR spectra obtained from these voxels, exhibiting notable differences: In the nucleus, the nonwater signal was very small, whereas large peaks typical of triglycerides could be observed in the animal and vegetal cytoplasm-they represent methine, the glycerol backbone, methylene, and methyl residues, as indicated in the figure
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Dissecting the vibrational entropy change on protein/ligand binding: burial of a water molecule in bovine pancreatic trypsin inhibitor
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5 the protein conformation undergoes a reversible transition which leads to a swelling of the monomeric unit (29,30)
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Similarly, repetitive T-jumps with DMPC disks containing apoA-1 and apoC-1 show faster unfolding in the second and consecutive jumps (data not shown)
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Tharp & young on ice creamQ What mix processing variables impact the eating quality of ice cream?A Unit operations tip to, but not including whipping and freezing, are considered mix processing
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Another recent, theoretical, contribution has pointed out that fine-structure of a few k^sub B^T within the transition state on a reaction pathway can accelerate folding (Wagner and Kiefhaber, 1999)
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This information is needed to locate main reproductive and nursery grounds for the species
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Configurational entropy changes per residueChanges in conformational entropy upon binding can also be calculated per residue
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Research elsewhere [1, 10, 12, 13] has shown that such gels form with a thermal phase inversion of polypropylene solutions in a hydrocarbon solvent when entering the metastable region of the phase diagram upon cooling
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Consistent with this hypothesis, previous trFRET studies have shown that the 4WJ stabilizes the tertiary structure of the hairpin ribozyme by reducing the entropie penalty for docking as compared with ribozyme constructs containing either a 2WJ or 3WJ (Klostermeier and Millar, 2000)
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One of the most dangerous is OxyContin, a pill that is designed to deliver pain relief over a 12-hour period
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He supports his negative critique by drawing detailed attention to several cases in point such as the pseudo-scientific nature of most cancer research, birth defects due to thalidomide and the vivisective approach to surgical training
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Characteristics and beliefs associated with probable pathological gambling: A pilot study with implications for the national gambling impact and policy commission
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When partition by age of legal wager, 9
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. Since the frequency of the solvent-exposed secondary structures is smaller than the buried ones (44,45), the above sequential events could be interpreted as follows: the unfolding of the solvent-exposed β-sheet structures is the earliest event, followed by the formation of aggregates, and finally the unfolding of the other native structures
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. However, with respect to the heating rate dependence of the respective λ^sub max^-values obtained for unfolded states, TAKA exhibits the same behavior as compared to the other α-amylases (Fig
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. The fluorescence emission of the folded state is already rather blue shifted (λ^sub max^ = 327 nm), but also rather large λ^sub max^-values (341-344 nm) for the unfolded states contribute significantly to the large red shift
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