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In the present study, we have investigated the equilibrium unfolding of yeast glutathione reductase (GR) by guanidine hydrochloride (GdnHCl)
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The interaction of a naphthalene sulfonate dye with apomyoglobin and apohemoglobin: a fluorescent probe of non-polar binding site
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The decreased strength of hydrophobic interactions at low temperature and the peculiarly high solute concentration (~5 M) and ionic strength of the liquid water pool may be factors that contribute to the lower affinity of ANS for proteins in ice
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Besides picosecond motions, all residues also experience internal motion with an effective correlation time of ~2
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In this study at pH 7
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1 is used) assuming the apparent specific volume of the protein phase to be 1
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However, repeating this study for solubilized proteins that were freshly dialyzed to remove detergent gave an apparent K^sub d^ only 10-fold weaker than predicted, suggesting that we may be not be viewing a simple protein-protein association (K^sub d^ ~ 70 nM; Fig
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Regulation of phosphoinositide-specific phospholipase C
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coli lac repressor-operator interaction: kinetic measurements and conclusions
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McLeishDepartment of Physics and Astronomy and Astbury Centre for Molecular Biology, University of Leeds, Leeds, United KingdomSubmitted November 4, 2003, and accepted for publication September 7, 2004
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The T^sub g^ of the EVA copolymers decreased with increasing vinyl acetate content
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In species with age-structured spawning schedules, a broad age distribution will maximize the length of the spawning season
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Forinformation on the latest finding, visit www
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"It's a wonderful idea" says Abramson
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Remington; the science and practice of pharmacy, 21st edRemington; the science and practice of pharmacy, 21st ed
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, but an officer who was there within seconds had to wait until help arrived from the Houston County Sheriff's Office, Minnesota Highway Patrol and Winona County Sheriff's Office, police Chief Todd Nelson said
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A machine made by Distributed Delivery Networks was installed at Kmart's busy Penn Station store in Manhattan last month, and depending on the test, this service could eventually be rolled out to Kmart's more than 1,000 pharmacies
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In making a decision to hit, players place the responsibility of loss (caused by a bust) on themselves, but, if they sit, any losses they experience can be attributed to factors beyond their control (i
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It was emphasized that the player should try to win as many points as possible
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Whereas Wolfgang, Zenker, and Viscusi (1984) and Ladouceur, Tourigny, and Mayrand (1985) reported evidence supporting Langer's model, Ladouceur, Mayrand, Dussault, Letarte, and Tremblay (1984) found that neither participation (the participant or the experimenter throwing a die) nor the level of involvement (variable, constant, or zero bet) affected the reported illusion of control
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- * Improving charge capture in clinical and ancillary areas is often recommended for increasing cash flow
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. The large-scale motion generated by backbone pivot moves ensures a lack of correlation between saved snapshots
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. the tertiary structure of EI^sup sc^ remained unaltered, as suggested by fluorescence and ANS-binding (Fig
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. Furthermore, studies with solubilizing agents such as detergents, reversed micelles, or nondetergent sulfobetaines (NDSB) proved that aggregation, which typically occurs during thermal unfolding in pure buffers, is strongly suppressed when such agents are added to the solution (29,32)
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.The influence of small differences in primary and quaternary structure and in degree of glycosylationDifferences in primary and quaternary structure, degree of glycosylation, and oligomeric state, between DmAChE and HuBChE, although they affect various structural and functional parameters, are not reflected in changes in global dynamics on an Ångström-nanosecond scale in the temperature range investigated
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. We have developed a method for analyzing ^sup 15^N-spin relaxation measured at two magnetic fields that corrects (τ^sup B^^sub m^)^sub app^ for internal motion up to ~4 ns, giving (τ^sup o^^sub m^)^sub app-corr^ (22)
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