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. , nativelike secondary structure and fluctuating tertiary structure) in different proteins
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. Secondary structure of proteins from circular dichroism spectra
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. ACYI has been successfully applied in the industry to produce L-amino acids (14,15)
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. Inasmuch as sequence-specific effects are present over five- to nine-residue stretches, it is conceivable that there are native-like local biases as well as default biases for conformations such as polyproline II (48)
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. Circular dichroism CD spectra in the far-UV region (200-280 nm) were recorded on a Jasco J-810 equipped with peltier thermostating cuvette holder under constant nitrogen flow
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. The presence of Mg^sup 2+^ slightly increased the T^sub m^ of EI^sup sc^ alone, probably due to formation of an octahedral-coordination-geometry complex to the native state of EI^sup sc^, as occurs in other homologous proteins (56,57)
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