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Steady-state and quenching experiments were acquired at 298 K
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We can speculate, however, based on the modeled three-dimensional structure of the EIC^sup sc^
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Infrared evidence of a β-hairpin peptide structure in solution
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Protein concentration was determined from the absorption at 280 nm and enzymatic activity was tested before and after neutron scattering experiments (36) at 25°C in a 25-mM phosphate buffer (pH 7
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With increasing attraction, three dimensional particle networks, with solidlike characteristics (colloidal gels), form at significantly lower particle volume fractions (10)
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After the buffer baseline subtraction, the data were normalized to protein concentration and expressed as molar residue duplicity, [Θ], No changes in [Θ] were detected with changes in the protein concentration in the range explored (5-600 µg/ml)
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. 1, are turned off, we obtain Flory's freely rotating chain model (43), albeit with a constraint that the peptide units are all in a trans configuration
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. In their original work, Plaxco et al
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.2 K; 4 µM, T^sub m^ = 329
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. Nonwetting occurs for the Ni substrate when R flux is used, because this type of flux contributes lower values of γSF - γSL and is attributed to the higher contact angles as well as negative wetting forces
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.Characterization of CPM-PLCβ-Gβγ associations at atmospheric pressureIn Fig
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. The structure of the ternary complex of the core of gp120, CD4, and a CD4-induced antibody is the only available atomic information to date (Kwong et al
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. Bonvin** Bijvoet Center for Biomolecular Research, Utrecht University, Utrecht, The Netherlands; and [dagger] Laboratory of Physical Chemistry, Swiss Federal Institute of Technology, Zurich, SwitzerlandSubmitted April 23, 2004, and accepted for publication September 20, 2004
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