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Structural interpretation of pH and salt-dependent processes in proteins with computational methods
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Reassessing random-coil statistics in unfolded proteins
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Mouse acetylcholinesterase unliganded and in complex with huperzine A: a comparison of molecular dynamics simulations
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Firstly, R^sub ex^ was derived from the ratio of R^sub 2^ values measured via a CPMG experiment at two different magnetic fields using the PINATA method
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6 when the protein has a high net positive charge (dimer > +36e) are lower, indicating the presence of attractive forces and aggregation processes under these conditions
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Although the fluorescence lifetime of ANS is not in the range to enable accurate determination of the rotational correlation time of a large oligomer, ANS anisotropy decay can be used as a measure of aggregation both through point measurements and in a kinetic assay showing limiting anisotropy baselines
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The labeling reaction was quenched after 10 min by the addition of 20 mM β-mercaptoethanol
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6; under these conditions, the protein self-association is minimal and the predominant apoA-2 form is a disulfide-linked dimer (Swaney and O'Brien, 1978; Donovan et al
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Furthermore, the bi-exponential heat-unfolding kinetics observed in apoA-2: DMPC disks (Fig
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A second consequence of this high-dimensional viewpoint is therefore that in some cases it might be fruitful to look for tuned but nonnative, interactions between sections of partially structured chain that stabilize intermediate search spaces
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Malin constructed the color image from three black-and-white negatives of CG4 taken through different filters
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Table 4Results of analyses of covariance testing for differences in slopesand elevations of annual absolute fecundity-age relation and annualrelative fecundity-age relation
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With fluorescence microscopy with B-exciting light, yellowish fluorescein coronas around the cells of the toxic species were observed, confirming a positive reaction
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