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. Denatured states figure prominently in a variety of studies on proteins especially as reference states for estimating protein stability (4,5,13-18)
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. The peptide still interacts with the same exposed hydrophobic patches as in the wraparound binding mode, but here a number of weak interactions occur instead of one strong
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. Purified proteins were dissolved in 8 M Urea and refolded by dialysis as described in Gursky and Atkinson (1996)
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