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. Average shapes are consistent with prolate ellipsoids
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. For reasons to be discussed below, this assertion is in fact inaccurate
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. However, the mean-field Flory random-coil model is not well-suited for explaining the source of scale-invariant behavior of denatured proteins
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. Protein concentration was calculated by using the extinction coefficients of Trp, Tyr, Cys, and Phe residues (24)
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. Most of these difficulties are not present if λ^sub max^-signals are employed to characterize unfolding transitions, although these signals can give a skewed assignment of the population of microstates, because a priori the λ^sub max^-signal is not proportional to the population of the states
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. This was possible due to the exploration of a novel pathway of TTR aggregation from the molten globule A-state, affording soluble assemblies of misfolded protein molecules
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. Consistent with previous observations (26), the intensity of the intrinsic Trp fluorescence decreased with the increase of temperature, and meanwhile, the emission maximum wavelength red shifted from ~333 nm to 340 nm (Fig
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. The observations that so many eukaryotic proteins are glycosylated and that N-linked glycosylation is more frequently observed in proteins suggest that N-linked glycans are a prime requisite for sustaining the structure-function equilibrium in many proteins (6)
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. The concentration of ANS, as determined by absorbance at 370 nm, using ε^sub 370^ = 6800 M^sup -1^Cm^sup -1^ (in methanol), ranged between 10 and 450 µM although in most experiments the upper limit was 320 µM
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