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. The fully deuterated samples were obtained by incubating the protein solutions in a water bath at 50°C for 15 min, and then the samples were cooled to room temperature and lyophilized
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. For sequence separations that go beyond this local length scale, chain properties such as R^sub g^
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. If R is the ANS/azurin molar ratio near or above the saturation point and M the corresponding fluorescence enhancement factor, M = F^sub max^/F^sub control^, then n = 0
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. Apart from the faster kinetics, the increased temperature of 70°C leads to a higher degree of morphological variability: along straight rods, twisted ribbonlike structures, rod bundles, and ropelike structures become prominent in the corresponding AFM data
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