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. on a typical trip, Hatfield and his crew don't suffer from a lack offish: Working the controls of the massive hydraulic winches, Hatfield winds a 220-foot-long green nylon net aboard
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. But why lose them? What's more, since so many species of cave-dwelling fish, not at all closely related, have lost their eyes, the phenomenon seems to be neither an accident devolution nor an isolated event
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. Thermodynamics of dcnaturant-induced unfolding of a protein that exhibits variable two-state denaturation
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. (59) Similar to statins, PPAR[gamma] agonists may also increase efferocytosis in the inflamed lung by decreasing oxidant stress, TNF-[alpha], and matrix metalloproteinases
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. A similar difference in emission peak maxima between thermal and GndHCl-induced unfolding was also observed for other proteins (see for example (10,21))
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. As a first step in this direction, we measured the thermal unfolding transition of α-amylases in the presence of NDSB, which is known to be an effective agent to prevent aggregation of thermal unfolded proteins (32-34)
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. In the large oocytes of the frog Xenopus laevis, this was small enough to allow the recording of the first compartment-selective in vivo MR spectra from the animal and vegetal cytoplasm as well as the nucleus
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. Thus, in contrast to subcellular MRI, we are not aware of any report of subcellular MRS
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. Delivery of drugs across cellular membranes is one of the main goals of current pharmaceutical research (43), including MRI contrast agent development for biological applications (44)
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. 3-5, the slope increases with increasing k^sup -1^^sub ex^ (see below; Fig
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. This is consistent with the EM data showing formation of large liposomes upon increasing the time and/or temperature of the sample incubation (Fig
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. but not LpA-2, provide substrates for LCAT, formation of LpA-1/A-2 may be facilitated by the relative ease of spontaneous LpA-2 fusion
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. After the incubation, VECTASTAI[N
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. Variation in digestion of prey by the Tawny Owl (Strix aluco)
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. The relationship observed between reduced protein flexibility and increased thermal stability is in line with other experiments on trehalose-coated myoglobin (56) and on lysozyme in glycerol (57,58)
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. This is in agreement with MD simulation data: Modifications in the dynamics of large protein complexes relative to their component subunit are dominated by low-frequency collective modes which take place on the 10-ps range upwards (2,49), and may be invisible in the experimental space-time window chosen
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. 5, C and D) and shorter fluorescence lifetimes (data not shown) reveal Trp to ANS and Bis-ANS fluorescence resonance energy transfer both in the native and misfolded states, indicating that the Tips and the probes are in close proximity (Conrad and Brand, 1968)
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. The amphipathic helix in the exchangeable apolipoproteins: a review of secondary structure and function
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. Denaturing urea gel electrophoresis followed by quantification of RNA bands using phosphorimager analysis and determination of the ratio of cleaved to uncleaved substrate strand as a function of time provided ribozyme activity information
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