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. GR is a 108-kDa, homodimeric flavoenzyme that catalyzes the [beta]-nicotinamide-adenine dinucleotide phosphate (NADPH)-dependent reduction of oxidized glutathione (GSSG) to its reduced form (GSH), thus participating in cellular defense mechanisms against oxidative stress
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. It is interesting to consider what the possible impact of FAD and NADPH would be on the folding of GR
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. Internal distances between residues that are beyond l^sub s^ should show the same power law dependence on sequence separation as does R^sub g^ (or R^sub e^) on chain length (N)
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. The large-scale motion generated by backbone pivot moves ensures a lack of correlation between saved snapshots
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. Structure and stability of EI^sup SC^ in the presence of the effectors All the experiments in the presence of PEP and Mg^sup 2+^ were carried out at pH 7
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. In all fluorescence experiments the samples were prepared from ANS and protein stocks to a final constant protein concentration of 2 µM in 2 mM Hepes buffer, pH 7
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. TheoryThe equations for the relaxation of a backbone amide ^sup 15^N spin in a protein as well as their interpretation in terms of the model-free approach of Lipari and Szabo (25) are well described in the literature (see, e
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. The effect of temperature and ionic strength on the dimerisation of beta-lactoglobulin
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. For the WT ribozymes, docked fractions (F) were calculated as a ratio of docked/undocked areas (A) as, F = A^sup docked^/(A^sup docked^ + A^sup undocked^)(Deniz et al
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. To confirm these assignments, we examined a destabilized 4WJ ribozyme variant, in which G^sub 11^ is replaced by inosine (I)
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. Faculty members' perceptions of student academic stress and reactions to stressors were higher than students' self-perceptions
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. The best data to date on gambling by college students is contained in a meta-analytic review of studies which surveyed college students in both the United States and Canada (Shaffer, Hall, & Bilt (1997)
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