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. In this work, we set n = 14
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. In addition, a 10-Å distance-based cutoff was applied between all nonbonded atoms
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. Indeed, for all 23 sequences in the EV limit we find that the absolute contact orders are independent of sequence
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. Steady-state intrinsic fluorescence Protein samples were excited at 280 and 295 nm between pH 2-12
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. Two pathways converge at CED-10 to mediate actin rearrangement and corpse removal in C
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. 5 a that the λ^sub max^-values obtained for the native and most probably also for the nonaggregated thermal unfolded state are strongly biased by direct interaction with NDSB
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. 5, A and B, show a correlation between the protein folding/unfolding and the changes in the particle morphology
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. The results of ANCOVA (Table 4) over a common age range showed no evidence of differences in slopes among the years 1996-98 (P=0
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. The rate constants were expressed in units of an experimental reference time, the average burst duration, as discussed later
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- They remained married until a heart attack claimed her life in May of this year
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. maxima GR by GdnHCl has been proposed (Rendon et al
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. The column was then washed extensively with PBS till the washings had A^sub 280^Isothermal guanidinium-hydrochloride-induced denaturation Equilibrium unfolding as a function of guanidinium hydrochloride (GdnCl) concentration was monitored by fluorescence spectroscopy
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. Some efferocytosis receptors appear to perform primarily a tethering function by holding the apoptotic cell and phagocyte close together (eg, CD14 or CD31), (19,21,22) while other receptors are primarily involved with transducing the engulfment signal (eg, LRP and PS receptors [PSRs]), (11,21,23,24) hence the so-called tether-and-tickle hypothesis (Fig 1, top, A)
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. Since the dealkylated soman moiety bound to the HuBChE monomer subunit contributes only three hydrogen atoms (P-CH^sub 3^) to the enzyme-inhibitor conjugate, while the deglycosylated native enzyme monomer itself accounts for >3500 nonexchangeable hydrogen atoms, the contribution of the inhibitor is negligibly small
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. We conclude that HuBChE stabilization by covalent modification with soman is due to an increase in the free energy of the unfolded state
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.CONCLUDING REMARKSGiven the severity of many diseases associated with protein misfolding and aggregation, including the amyloidoses and the transmissible spongiform encephalopathies, research in this area is intense
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. Membrane binding of the G-protein subunits was followed by changes in the fluorescence of the CPM-labeled proteins (see Philip and Scarlata, 2004)
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. This apparent K^sub d^, which reflects protein-protein associations on a confined membrane surface, is predicted to be ~2 orders of magnitude weaker when the proteins are free to associate in solution (see Runnels and Scarlata, 1999)
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. It shares with the present treatment the essential departure from a one-dimensional projection of a transition state, and an identification of the number of permissible pathways, or transition entropy, in controlling the rate of folding
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