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Two intermediate states were populated at 2-3 M GdnHCl, as indicated by a combination of spectroscopic and chromatographic methods
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The latter is a model for a soft, Gaussian coil with large-scale, albeit uncorrelated
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Given the equivalence between the EV limit and highly denatured states, our ability to simulate conformational ensembles in the EV limit, with full atomic detail, has direct bearing on the development of an accurate physical picture of conformations accessible to denatured proteins
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The whole emission spectrum with a wavelength increment of I nm was acquired approximately within 30 s
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Strambini, unpublished data) provides additional evidence that the creation of these low affinity ANS binding sites is owed to perturbations of the protein fold associated with the frozen medium
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The intraresidue conformational entropy obtained in this way provides, therefore, only information about side-chain motions
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The fabrics in this study are described in Table II
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The influence of cooling conditions on particle size and shape is summarized in Table V
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