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. Moreover, the strategies used in this research might also facilitate the characterization of the potential pretransitional changes in the thermal transitions of other thermostable or thermophilic proteins
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. Address reprint requests to Avadhesha Surolia, National Institute of Immunology, Aruna Asaf Ali Marg, New, Delhi-110067, India
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. In the EV limit, there exists a so-called "correlation hole" (39)
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. Among a large variety of different methods, such as absorption-, circular dichroism spectroscopy, calorimetry, NMR, and light scattering techniques, in particular fluorescence spectroscopy is a powerful technique to monitor unfolding transitions and to characterize properties of the unfolded state (see for example (3,4))
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. 15 M Tris-HCl, pH 8, followed by column chromatography (23)
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. The similar T^sub 2^ values of the animal and vegetal methylene peaks imply that molecular motion occurs at the same timescale in both compartments and cannot account for the linewidth difference
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. The stabilization of state C is probably due to an increase of its entropy, related to larger MSDs (in line with neutron scattering experiments on denatured α-amylase, (51))
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. These channels remain open for the Sn-Ag-Cu-Bi solder, whereas they are closed for the Sn-Pb solder at a certain period
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. Thus, it becomes possible to identify P
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. The efficiency E of this process exhibits a strong distance dependence, given by E = 1/(1 + (R/R^sub 0^)^sup 6^), where R is the donor-acceptor distance and R^sub 0^ is the Förster radius at which the efficiency is 50%
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. The pellets were scattered beneath a large black walnut tree (Juglans nigra Linné, 1753) along the edge of a windbreak of trees bordering a grassy, overgrown field just north of Old Church Road and west of Route 183
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. ((Comments on this story may be sent to aii
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. The percent of underage students who reported having gambled in a casino is consistent with results found in previous studies both at UNLV and at other campuses located near gaming opportunities (Knapp & Crossman, in preparation; Stitt, Giacoppassi, & Vandiver, 2000)
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. Due to its important physiological roles in intracellular amino acid metabolism, ACYI deficiency caused by genetic mutation has recently been related to inborn metabolism diseases (12,13)
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. The quality of the final products was characterized to be homogeneous on size exclusion chromatography and polyacrylamide gel electrophoresis in the presence and absence of SDS, and the purity of the protein was estimated to be above 98%
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. Positive crosspeaks could be found at (1649 cm^sup -1^, 1636 cm^sup -1^), and negative crosspeaks were located at (1649 cm^sup -1^, 1617 cm^sup -1^) and (1658 cm^sup -1^, 1617 cm^sup -1^)
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. Values of R^sub o^ and ν will vary with solution conditions
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. To calculate the form factor we used the positions of α-carbon atoms for each residue
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. The far-UV CD spectrum of EI^sup sc^ at neutral pH showed the features of an α-helical protein with minima at 208 and 222 nm (41,42) (Fig
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.669 µm for Sn-Ag-Cu-Bi solders) compared to those of the Cu substrates
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. The backbone atoms (N, Cα, and C') of the most stable structural elements of both proteins, the first α-helix of gp120 (residues 17-31), and part of the β-sheet of CD4 consisting of residues 26-30 and 82-86 (see Fig
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