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. DISCUSSIONMolten globule (MG) folding intermediates have been characterized for a number of proteins (for reviews, see Kuwajima, 1989; Ptitsyn, 1992, 1995)
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. Synthesis of soybean agglutinin in bacterial and mammalian cells
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. At pH 4, the sample precipitated, and at pH 10, no endotherm was observed
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. For example, problems arise from the intrinsic temperature dependence of the tryptophan fluorescence emission and from a pronounced protein concentration dependence, which leads to a significant scattering in the data
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. Istituto di Biofisica Area della Ricerca, San Cataldo via G
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. It should be noted, however, that B-factors from x-ray crystallography, in general, include contributions from static disorder, and care must be taken in comparing them to thermal atomic fluctuations (14)
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. The entire tests were performed with the solder bath temperatures of 255°C, 275°C, and 295°C, where the dwell time was 10 sec
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. A further note of importance is that for the CPMG settings used, R^sub 2^(δ^sub 1^) is measured in the high ω^sub 1eff^ limit (ω^sub 1eff^ > p^sub A^Δω^sub I^), so that R^sub ex^ is proportional to B^sup 2^^sub o^ independent of whether the exchange is fast or slow to a skewed population (33,34)
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. DISCUSSIONThe dimeric CaM:SEF2-1mp complex shows unique dynamical behavior
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. To calculate the protein number density, the dimer molecular mass of 36
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. This is not yet reachable for proteins with the current computation timescale of nanoseconds
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. 0001,) of a stage effect (i
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. )19Fisheries Laboratory Late15 Jan '97of Kinki University(Kushimoto, Wakayama Pref
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