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. Although gSBA was seen to demonstrate very high conformational stability, rSBA exhibits comparatively much lower firmness to resist the structural perturbation induced by the denaturant guanidinium hydrochloride
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. Simply stated, chain properties for long chains can be predicted by scaling the corresponding properties for short chains and vice versa
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. However, our data indicate that aggregation and in addition at least one of these other mechanisms seems to play a role for the α-amylases with red shifts upon thermal unfolding (i
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. Several general predictions result: 1), intermediate subspaces of configurations will always be visited; 2), specific but nonnative interactions may be important in stabilizing these low-dimensional diffusive searches on the folding pathway, as well as native interactions; 3), sequential barriers will commonly be found, even in "two-state" proteins; 4), very early times will show characteristic departures from single-exponential kinetics; and 5), contributions of nonnative interactions to Φ-values and "Chevron plots" are calculable, and may be significant
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. The rate-determining "gutter" dimensions lie orthogonal to other "zipper" dimensions describing the local formation of secondary structure, that are characterized by a continuous folding funnel
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